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Saccharopolyspora oryzae: A Novel Actinobacterium Found in Rice Rhizosphere Soil

A new actinobacterium, named Saccharopolyspora oryzae, has been discovered in the rhizosphere soil of rice plants. The strain, designated as WRP15-2T, is Gram-stain-positive, aerobic, and non-motile.

Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain WRP15-2T belongs to the genus Saccharopolyspora. It showed the highest similarity (99.1%) to Saccharopolyspora kobensis JCM 9109T, followed by Saccharopolyspora indica VRC122T (98.9%) and Saccharopolyspora antimicrobica DSM 45119T (98.7%). However, further analysis using digital DNA-DNA hybridization and average nucleotide identity confirmed that strain WRP15-2T represents a novel member of the Saccharopolyspora genus.

Chemotaxonomic data indicated that strain WRP15-2T predominantly contains MK-9(H4) as its menaquinone. It also contains meso-diaminopimelic acid as the diagnostic diaminopimelic acid and arabinose, galactose, and ribose as the major whole-cell sugars. The detected phospholipids were mainly phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxy-phosphatidylmethyleth

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